Optogenetics uses light sensitive proteins called opsins to modulate and monitor the activity of neurons and other cellular states. We are working to extend the properties of opsins through protein engineering.
Gradinaru lab publications in PubMed and bioRxiv.
2021
Light-guided sectioning for precise in situ localization and tissue interface analysis for brain-implanted optical fibers and GRIN lenses
Sep 28, 2021, Cell Rep
Kahan A, Greenbaum A, Jang MJ, Robinson JE, Cho JR, Chen X, Kassraian P, Wagenaar DA, Gradinaru V.
Age-dependent alterations in key components of the nigrostriatal dopaminergic system and distinct motor phenotypes
July 09, 2021, Acta Pharmacologica Sinica
Jiang-peng Fan, Hui-zhen Geng, Ya-wei Ji, Tao Jia, Jennifer B. Treweek, An-an Li, Chun-yi Zhou, Viviana Gradinaru & Cheng Xiao
Dorsal raphe dopamine neurons signal motivational salience dependent on internal state, expectation, and behavioral context
February 9, 2021, Journal of Neuroscience
Jounhong Ryan Cho, Xinhong Chen, Anat Kahan, J. Elliott Robinson, Daniel A. Wagenaar and Viviana Gradinaru.
2020
Directed Evolution of a Selective and Sensitive Serotonin Sensor via Machine Learning
December 16, 2020, Cell
Elizabeth K. Unger, Jacob P. Keller, Michael Altermatt, Ruqiang Liang, Aya Matsui, Chunyang Dong, Olivia J. Hon, Zi Yao, Junqing Sun, Samba Banala, Meghan E. Flanigan, David A. Jaffe, Samantha Hartanto, Jane Carlen, Grace O. Mizuno, Phillip M. Borden, Amol V. Shivange, Lindsay P. Cameron, Steffen Sinning, Suzanne M. Underhill, David E. Olson, Susan G. Amara, Duncan Temple Lang, Gary Rudnick, Jonathan S. Marvin, Luke D. Lavis, Henry A. Lester, Veronica A. Alvarez, Andrew J. Fisher, Jennifer A. Prescher, Thomas L. Kash, Vladimir Yarov-Yarovoy, Viviana Gradinaru, Loren L. Looger, and Lin Tian.
Negative feedback control of neuronal activity by microglia
September 30, 2020, Nature
Ana Badimon, Hayley J Strasburger, Pinar Ayata, Xinhong Chen, Aditya Nair, Ako Ikegami, Philip Hwang, Andrew T Chan, Steven M Graves, Joseph O Uweru, Carola Ledderose, Munir Gunes Kutlu, Michael A Wheeler, Anat Kahan, Masago Ishikawa, Ying-Chih Wang, Yong-Hwee E Loh, Jean X Jiang, D James Surmeier, Simon C Robson, Wolfgang G Junger, Robert Sebra, Erin S Calipari, Paul J Kenny, Ukpong B Eyo, Marco Colonna, Francisco J Quintana, Hiroaki Wake, Viviana Gradinaru, Anne Schaefer
Dorsal raphe dopamine neurons signal motivational salience dependent on internal and external states
July 27, 2020, BioRxiv
Jounhon Ryan Cho, Xinhong Chen, Anat Kahan, J. Elliott Robinson, Daniel A. Wagenaar, Viviana Gradinaru
RecV recombinase system for in vivo targeted optogenomic modifications of single cells or cell populations
March 23, 2020, Nature Methods
Shenqin Yao, Peng Yuan, Ben Ouellette, Thomas Zhou, Marty Mortrud, Pooja Balaram, Soumya Chatterjee, Yun Wang, Tanya L. Daigle, Bosiljka Tasic, Xiuli Kuang, Hui Gong, Qingming Luo, Shaoqun Zeng, Andrew Curtright, Ajay Dhaka, Anat Kahan, Viviana Gradinaru, Radosław Chrapkiewicz, Mark Schnitzer, Hongkui Zeng, Ali Cetin
2019
Machine learning-guided channelrhodopsin engineering enables minimally invasive optogenetics
October 14, 2019, Nature Methods
Claire N. Bedbrook, Kevin K. Yang, J. Elliott Robinson, Elisha D. Mackey, Viviana Gradinaru, Frances H. Arnold
The Serotonergic Raphe Promote Sleep in Zebrafish and Mice
June 24, 2019, Neuron
Grigorios Oikonomou, Michael Altermatt, Rong-wei Zhang, Gerard M. Coughlin, Christin Montz, Viviana Gradinaru, David A. Prober
Identification of peripheral neural circuits that regulate heart rate using optogenetic and viral vector strategies
April 26, 2019, Nature Communications
Pradeep S. Rajendran, Rosemary C. Challis, Charless C. Fowlkes, Peter Hanna, John D. Tompkins, Maria C. Jordan, Sarah Hiyari, Beth A. Gabris-Weber, Alon Greenbaum, Ken Y. Chan, Benjamin E. Deverman, Heike Münzberg, Jeffrey L. Ardell, Guy Salama, Viviana Gradinaru, Kalyanam Shivkumararticle
2017
Deep tissue optical focusing and optogenetic modulation with time-reversed ultrasonically encoded light
December 8, 2017, Science Advances
Haowen Ruan, Joshua Brake, J. Elliott Robinson, Yan Liu, Mooseok Jang, Cheng Xiao, Chunyi Zhou, Viviana Gradinaru, Changhuei Yang
Overriding sleep
October 26, 2017, Science
Viviana Gradinaru
Machine learning to design integral membrane channelrhodopsins for efficient eukaryotic expression and plasma membrane localization
October 23, 2017, PLOS Computational Biology
Claire N. Bedbrook, Kevin K. Yang, Austin J. Rice, Viviana Gradinaru, Frances H. Arnold
Q&A: How can advances in tissue clearing and optogenetics contribute to our understanding of normal and diseased biology?
September 25, 2017, BMC Biology
Alon Greenbaum, Min J. Jang, Collin Challis, Viviana Gradinaru
Dorsal Raphe Dopamine Neurons Modulate Arousal and Promote Wakefulness by Salient Stimuli
June 8, 2017, Neuron
Jounhong Ryan Cho, Jennifer B. Treweek, J. Elliott Robinson, Cheng Xiao, Lindsay R. Bremner, Alon Greenbaum, Viviana Gradinaru
Structure-guided SCHEMA recombination generates diverse chimeric channelrhodopsins
March 10, 2017, Proceedings of the National Academy of Sciences
Claire N. Bedbrook, Austin J. Rice, Kevin K. Yang, Xiaozhe Ding, Siyuan Chen, Emily M. LeProust, Viviana Gradinaru, Frances H. Arnold
Directed Evolution of a Bright Near-Infrared Fluorescent Rhodopsin Using a Synthetic Chromophore
March 2, 2017, Cell Chemical Biology
Lukas Herwig, Austin J. Rice, Claire N. Bedbrook, Ruijie K. Zhang, Antti Lignell, Jackson K.B. Cahn, Hans Renata, Sheel C. Dodani, Inha Cho, Long Cai, Viviana Gradinaru, Frances H. Arnold
Global Representations of Goal-Directed Behavior in Distinct Cell Types of Mouse Neocortex
May 17, 2017, Neuron
William E. Allen, Isaac V. Kauvar, Michael Z. Chen, Ethan B. Richman, Samuel J. Yang, Ken Chan, Viviana Gradinaru, Benjamin E. Deverman, Liqun Luo, Karl Deisseroth
2016
Fluorescent boost for voltage sensors
January 26, 2016, Nature
Viviana Gradinaru, Nicholas C. Flytzanis
2014
Archaerhodopsin variants with enhanced voltage-sensitive fluorescence in mammalian and Caenorhabditis elegans neurons
September 15, 2014, Nature Communications
Nicholas C. Flytzanis, Claire N. Bedbrook, Hui Chiu, Martin K. M. Engqvist, Cheng Xiao, Ken Y. Chan, Paul W. Sternberg, Frances H. Arnold, Viviana Gradinaru
Directed evolution of a far-red fluorescent rhodopsin
August 25, 2014, Proceedings of the National Academy of Sciences
R. S. McIsaac, M. K. M. Engqvist, T. Wannier, A. Z. Rosenthal, L. Herwig, N. C. Flytzanis, E. S. Imasheva, J. K. Lanyi, S. P. Balashov, V. Gradinaru, F. H. Arnold